Journal: bioRxiv
Article Title: Single-cell Analysis of Paired FFPE Placentas Reveals Trophoblast Reprogramming and Immune Dysregulation in Chronic Villitis of Unknown Etiology
doi: 10.64898/2026.02.28.708431
Figure Lengend Snippet: (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. (H) Multiplex immunohistochemistry (mIHC) showing CK7 + (green) HLA-A/B + (magenta) trophoblasts in VUE tissue (white arrows).
Article Snippet: For multiplex immunohistochemical staining, primary antibodies are against human CK7 (17513, Proteintech), and HLA-A/B (15240-1-AP, Proteintech), the Four Detect Kit for Rabbit Primary Antibody kit (PK10033, Proteintech) used for multiple staining.
Techniques: Expressing, Control, Multiplex Assay, Immunohistochemistry